SIS3 HCl | TGF-beta/Smad 抑制剂 | 现货供应 | 美国品牌 | 免费...

来自 : www.selleck.cn/products/sis... 发布时间：2021-03-24

E-cadherin / -SMA ; PubMed: 29207055 Int J Mol Med
Co-localization of E-cad and -SMA occurs in A549 cells as determined by immunofluorescent staining. E-cad (green) was present in the cytoplasm, FITC-stained; -SMA (red) was present in the cytoplasm, TRITC-stained. Cell nuclei were counterstained with DAPI (blue). Scale bar, 40 m. The cell groups were as follows: control, cells cultured in serum-free DMEM; TGF- 1, cells cultured in serum-free DMEM and exposed to 5 ng/ml TGF- SIS3, cells were treated with 3 M SIS3 (specific inhibitor of Smad3) which was added 4 h prior to 5 ng/ml TGF- 1 exposure; BMSCs-CM, BMSCs-CM was added prior to 5 ng/ ml TGF- 1 exposure. E-cad, E-cadherin (E-calcium mucins); -SMA, -smooth muscle actin; TRITC, tetramethyl rhodamine isothiocyanate; FITC, fluorescein isothiocyanate; DAPI, 4 ,6-diamidino-2-phenylindole.

SIS3是一种新型的特异性Smad3抑制剂,通过抑制Smad3的磷酸化抑制TGF-β和activin信号,而不影响MAPK/p38, ERK或PI3-kinase信号通路。SIS3是一种新型的特异性Smad3抑制剂，通过抑制Smad3的磷酸化抑制TGF-β和activin信号，而不影响MAPK/p38, ERK或PI3-kinase信号通路。SIS3 HCl